¿Õ¼ä´úл×éѧ(Spatial Metabolomics)ÊÇ»ùÓÚÖÊÆ׳ÉÏñÊÖÒÕ¶ÔÑù±¾¾ÙÐдúл×éѧÆÊÎöµÄÒªÁ죬Äܹ»½«´úл×éѧÖÐÑù±¾´úлÎïµÄÖÖÀà¡¢º¬Á¿ÐÅÏ¢£¬½øÒ»²½À©Õ¹µ½¿Õ¼äˮƽÉÏ£¬ÊµÏÖ´Ó¶¨ÐÔ¡¢¶¨Á¿¡¢¶¨Î»Èý¸öά¶ÈÆÊÎöÉúÎï´úлÀú³ÌµÄ²î±ðת±ä¡£
´óÆøѹɨÃè΢̽Õë»ùÖʸ¨Öú¼¤¹â½âÎüµçÀëÖÊÆ׳ÉÏñ¼ì²â£¬Ê¹ÓûùÖʸ¨Öú¼¤¹â½âÎüµçÀëÊÖÒÕ(Minority Adoption Leadership Development Institute, MALDI)ÔÚ³£Ñ¹µ½ÖÐѹµÄ²Ù×÷ÇéÐÎÖÐÖ±½ÓɨÃèÉúÎïÑùÆ·³ÉÏñ£¬È»ºóͨ¹ýÖÊÆ×ÒDzⶨÀë×ÓµÄÖʺɱÈ(m/z)À´ÆÊÎöÉúÎï·Ö×ӵıê×¼·Ö×ÓÖÊÁ¿£¬²¢ÍŽáרÃŵÄÖÊÆ׳ÉÏñÈí¼þ¸¨Öú±¬·¢·Ö×ÓͼÏñ¡£¸ÃÊÖÒÕ²»µ«¿ÉÒÔ¶ÔÑù±¾ÖдúлÎïµÄ×é³É×é³É¡¢Ïà¶Ôº¬Á¿¾ÙÐÐÎÞ±ê¼Ç¼ì²â£¬»¹¿ÉÒÔÆÊÎö´úлÎïÔÚÑù±¾ÖеÄÌØÒìÐÔ¿Õ¼äÂþÑÜÐÅÏ¢¡£Òò´ËÔÚÁÙ´²Ò½Ñ§¡¢·Ö×ÓÉúÎïѧ¡¢Ö²Îïѧ¡¢¶¯ÎïѧµÈÁìÓò¾ßÓÐÖØ´óµÄÓ¦ÓÃÔ¶¾°¡£
¶¨ÐÔÆÊÎöµÄͬʱ£¬ÆÊÎöÎïÖʵĿռäÂþÑÜÌØÕ÷
¸ß¿Õ¼äÇø·ÖÂÊ¡¢¸ßÖÊÁ¿Çø·ÖÂÊ
²Ù×÷Á÷³Ì¼òÆÓ£¬ÊÊÓÃÐÔ¹ã
? Ñù±¾ÀàÐÍ
¶¯ÎïÑù±¾£ºÖ×Áö×éÖ¯£¬²î±ðÆ÷¹ÙÈçÐÄ¡¢¸Î¡¢ÄÔ¡¢·ÎµÈ
Ö²ÎïÑù±¾£ºÕûÖêÖ²Î»òÕ߸ù¡¢¾¥¡¢Ò¶¡¢ÖÖ×ÓµÈ
À¥³æÑù±¾£ºÕûÖ»À¥³æ£¬À¥³æÆ÷¹ÙµÈ
×éÖ¯ÇÐƬ£ºÍ¨ÀýÔز£Æ¬
? Ñù±¾×¼±¸Ö¸ÄÏ
¡ñ ÁÙ´²¡¢¶¯Îï¡¢Ö²Îï¡¢À¥³æÑù±¾µÈÑù±¾ÀàÐͶ¼Äܹ»¾ÙÐпռä´úл×éѧ¼ì²â¡£¿ÉÒÔÖ±½Ó½«ÍêÕûÑù±¾¼ÄËÍÀíÓÉÎÒÃÇÈÏÕæÇÐƬ£¬»òÕßÒ²¿ÉÒÔÌṩÖƱ¸ºóµÄÑù±¾ÇÐƬ¡£
¡ñ ×¢ÖØÑù±¾²»¿É¾Óɸ£¶ûÂíÁÖ½þÅÝ¡¢H&EȾɫ¡¢Ó«¹â±ê¼ÇµÈ´¦Öóͷ£¡£
¡ñ ÉúÎï×éÖ¯Ñù±¾¿ÉÒÔΪÀ䶳Ñù±¾»òÐÂÏÊÉúÎï×éÖ¯Ñù±¾¡£ÐÂÏÊÑù±¾½¨Òé¸É±ù-Õý¼ºÍéÔ¡ËÙ¶³ºó£¬ÒÔÂÁ²»òÀ䶳ÉúÑĹܣ¨Èç50mlÀëÐĹܣ©ÔÚ-80¡æ¾ÙÐÐÉúÑÄ¡£¼ÄËÍʱ½¨ÒéÑ¡Ôñ¼ÓºñµÄÅÝÄÏ䣬ʹÓÃ×ãÁ¿¸É±ù¼ÄËÍ£¬°ü¹ÜÑù±¾ËùÓÐÂñÔڸɱùµ±ÖУ¬ÉÏ϶¼ÓиɱùÁýÕÖ¡£
¡ñ Ö²ÎïÑù±¾¿ÉÒÔΪÕûÖê´øÍÁÐÂÏÊÑù±¾£¬»òÊÇȡϵÄÖ²ÎïÆ÷¹Ù£¬Èç¸ù¡¢¾¥¡¢Ò¶¡¢»¨µÈ£¬¿ÉÒÔÒÀ¾ÝÑù±¾ÇéÐÎÑ¡Ôñ³£Î¡¢±ù´ü»ò¸É±ù¼ÄËÍ¡£
¡ñ ͨÀýÑù±¾¼ì²âÃæ»ýÔ¼ÔÚ1cm2ÒÔÄÚ£¬ÈôÊÇÁè¼Ý¸ÃÃæ»ý¹æÄ££¬ÇëÏÈÁªÏµ×¤µØÏúÊÛ˾Àí¡£
? ÉúÎïѧÖظ´
Ñù±¾ÊýÄ¿£ºÖ²ÎïºÍ΢ÉúÎïn¡Ý5£¬¶¯ÎïÑù±¾n¡Ý3£¬ÁÙ´²Ñù±¾n¡Ý5£¬ËùÓÐÖظ´Ñù±¾×ÔÁ¦ÆÊÎö
- ÉúÃü¿Æѧ£ºÎïÖÊÔÚ×éÖ¯ÖеĿռäÂþÑÜ¿ÉÊÓ»¯
- ÁÙ´²Ò½Ñ§£ºÆÊÎöÖ×Áö¡¢ÐÄѪ¹Ü¼²²¡¡¢´úлÀàµÈ¼²²¡±¬·¢¼ÍÂÉ£¬Ñ°ÕÒÉúÎï±ê¼ÇÎï
- Ò©ÎïÑз¢£º·Ö×Ó»úÖÆ£¬×¼È·¶¨Î»Ò©ÎïÔÚ×éÖ¯ÄÚµÄÂþÑÜ
- Å©Òµ¡¢ÇéÐΡ¢Ö²Îﲡº¦¼°³¦µÀ΢ÉúÎïÏà¹ØµÄÑо¿ÁìÓò
? ʹÓÃAP - SMALDIÖÊÆ׳ÉÏñÕ¹ÏÖÓñÃ×ÖлÆÇúù¶¾ËØB1ºÍÖ²Îï·ÀÓù´úлÎïµÄ¿Õ¼äÂþÑÜ
Ñо¿¹¤¾ß£º ÓñÃ×
ÆÚ¿¯£º The Plant Journal
Ó°ÏìÒò×Ó£º 7.091
½ÒÏþʱ¼ä£º 2021Äê
? Ñо¿Åä¾°
»ÆÇúù¶¾ËØB1(AFB1)£¬ÊÇÓÉ»ÆÇúùºÍ¼ÄÉúÇúù±¬·¢µÄ×îÓÐÓúÍ×ºñµÄ´ÎÉú´úл²úÆ·¡£ÏÖÔÚµÄÑо¿¶ÔÓñÃ×ÊÜ»ÆÇúùѬȾʱ£¬Ö²Îï×éÖ¯´úлÎïת±ä£¬¶¾ËصÄÎüÊÕºÍÂþÑÜÏàʶÓÐÏÞ¡£±¾ÊµÑéͨ¹ýAP-SMALDI MSI£¬ÆÊÎöAFB1¶Ô¿µ½¡ÓñÃ×Ö²Öê³õ¼¶ºÍ´Î¼¶´úлµÄÉúÎïת»¯¡¢¶¨Î»ºÍºóÐøÓ°Ïì¡£¸ß¿Õ¼äÇø·ÖÂÊ(5?m)ÌṩÁËAFB1ÔÚ¸ùϸ°ûÖеÄϸÄ嶨룬ÒÔ¼°ÓÉAFB1»ýÀÛÔÚ²î±ðÆ÷¹ÙºÍ×éÖ¯ÖÐÓÕµ¼µÄÓñÃ×´úлת±ä¡£Æ¾Ö¤·Ç°ÐÏò´úл×éѧÑо¿£¬¸ùϵ»¨ÇàËØÉúÎïºÏ³ÉºÍÒ¶ÂÌËØ´úлÊÜAFB1»ýÀÛµÄ×ÌÈÅ×î´ó£¬¿ÉÄÜÓëϸ°ûÆÆËéËØ;¾¶Êܵ½ÒÖÖÆÓйء£
? Ñо¿Ë¼Ð÷
? ÎÄÏ×·ÖÏí
ighetti, L., Bhandari, D.R., Rolli, E., Tortorella, S., Bruni, R., Dall¡¯Asta, C. and Spengler, B. , Unveiling the spatial distribution of aflatoxin B1 and plant defense metabolites in maize using AP-SMALDI mass spectrometry imaging. Plant J, 106: 185-199 (2021)